Flow Cytometry Training

This streamlined, brand-neutral educational program is delivered as subtitled video-based content and simulation. Beginner and advanced users can take advantage of the extensive course content to gain a full understanding of fundamental concepts and advanced specialty topics. This program is also a great resource to prepare for the Specialist in Cytometry, SCYM (ASCP) exam.

PROGRAM OUTLINE

Check the program outline for an overview of the topics covered in each eCourse.
    • What is Flow Cytometry?
    • Common Applications
    • Fluorescence – Light Spectrum
    • Fluorescence – Absorption & Emission
    • Fluorescence – Fluorochrome Classes
    • Fluorescence – Fluorochrome Properties
    • Fluidics – Flow
    • Fluidics – Pressure & Flow Rate
    • Fluidics – Acoustic-Assisted Focusing
    • Optics – Excitation
    • Optics – Light Collection
    • Optics – Photodetection
    • Electronics – Amplification & Conversion
    • Electronics – Voltage Pulse
    • Electronics – Time Delay
    • Electronics – Digitization & Binning
    • Electronics – FCS File
    • Section Summary
    • Sample Requirements
    • Cellular Markers and Dyes
    • Fluorescence Detection
    • Fluorochrome Selection
    • Antigen Classification
    • Antibody Titration
    • Dead Cell Staining
    • Exclusion / Dump Channel
    • Spectral Overlap
    • Compensation Controls
    • Compensation
    • Spectral Overlap Reduction
    • Spillover Spreading
    • Spillover Spreading Matrix
    • Autofluorescence
    • Fc-Mediated Binding
    • Fluorescence Minus One Controls
    • Biological Controls
    • Replicates
    • Section Summary
    • Plots & Gates
    • Scaling
    • Acquisition Speed
    • Event Cycling
    • Scatter Setup
    • Threshold & Trigger
    • Aggregate Discrimination
    • Dead Cell Exclusion
    • Fluorescent Parameter Setup
    • Compensation
    • Data Recording
    • Data Exporting
    • Assay Setup Validation
    • Instrument Cleaning
    • Section Summary
    • Matrix Inspection
    • Data Display – Histograms & Plots
    • Data Display – Gates, Regions, Quadrants
    • Data Display – Gating
    • Biexponential Scaling
    • Data Cleaning – Time Gating
    • Data Cleaning – Debris Exclusion
    • Data Cleaning – Aggregates & Dead Cells
    • Data Cleaning – Autofluorescence
    • Data Cleaning – Antibody Aggregates
    • Data Cleaning – Impact
    • Gate Validation – FMO Controls
    • Gate Validation – Backgating/Color Gating
    • Cell Cycle – One-color DNA Analysis
    • Cell Cycle – Multi-color DNA Analysis
    • Section Summary
    • Number of Events to Record
    • Descriptive Statistics – Distribution
    • Descriptive Statistics – Central Tendency
    • Descriptive Statistics – Dispersion
    • Descriptive Statistics – Data Normalization
    • Descriptive Statistics – Fold Change
    • Inferential Statistics – Statistical Test
    • Inferential Statistics – Statistical Power
    • Inferential Statistics – Hypotheses
    • Inferential Statistics – Significance Level
    • Inferential Statistics – Statistical Errors
    • Inferential Statistics – Probability Value
    • Student’s t-test – T-distribution & Freedom
    • Student’s t-test – Formula
    • Student’s t-test – Real-Life Example pt1
    • Student’s t-test – Real-Life Example pt2
    • Section Summary
    • What is Cell Sorting? | Process Overview
    • Nozzle Size & Sheath Pressure | Droplet Generation
    • Droplet Breakoff
    • Stream Charging & Deflection
    • Collection
    • Instrument Optimization | Drop Delay
    • Acquisition & Gating
    • Conflicts
    • Sort Decisions
    • Sorting
    • Post-Sort – Purity, Recovery & Yield
    • Process Stressors | Sample Quality
    • Temperature & Pressure | Sample Media & Sheath
    • Collection Media | Instrument Variables
    • Sort Performance | Sort Quality
    • Collection Issues | Contamination
    • Single-Cell Deposition
    • Biosafety | Summary
    • Quiz (20 questions)
    • What is Full-Spectrum Flow Cytometry?
    • Full-Spectrum vs. Conventional
    • Spectral Emission Signature
    • Signature Comparison
    • Signature Alterations
    • Reference Controls
    • Spectral Reference Library
    • Instrument Settings
    • Acquisition
    • Spectral Profile & Unmixing
    • Autofluorescence Management
    • Unmixing Errors
    • Analysis
    • Panel Optimization – Introduction
    • Panel Optimization – Design
    • Panel Optimization – Testing
    • Panel Optimization – Evaluation & Remediation
    • Summary
    • Quiz (20 questions)